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KMID : 0360220090500081254
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Journal of the Korean Ophthalmological Society
2009 Volume.50 No. 8 p.1254 ~ p.1258
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Effect of Dexamethasone on the Production of Nitric Oxide in Trabecular Meshwork Cells
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Lee Su-Yoon
Kim Jae-Woo
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Abstract
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Purpose: To investigate the effects of dexamethasone (DEX) on the production of nitric oxide (NO) and its enzymatic synthetic pathway in cultured human trabecular meshwork (HTM) cells.
Methods: Primarily cultured HTM cells were exposed to 0, 10, 100, 1000 nM of DEX for 3 days. In addition, 100 μM sepiapterin, 100 μM ascorbic acid, and 10 μM methotrexate were co-exposed to DEX. The cellular survival and nitrite production rates were assessed by MTT assay and Griess assay, respectively.
Results: DEX did not significantly affect the survival of cultured HTM cells. DEX decreased the NO production in a dosedependent manner. With co-exposure of DEX, ascorbic acid nullified the DEX-induced decrease of NO production. Sepiapterin and methotrexate did not affect DEX-induced decrease of NO production.
Conclusions: DEX decreased NO production in HTM cells and the de novo pathway of tetrahydrobiopterin may be involved. This decrease may raise intraocular pressure by decreasing trabecular outflow.
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KEYWORD
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Dexamethasone, Nitric oxide, Tetrahydrobiopterin, Trabecular meshwork cells
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